Laboratory and Bio-repository Facilities at Centre for Chronic Disease Control in collaboration with Public Health Foundation of India

Need for an in-house laboratory

  • Quality assurance
  • Cost effectiveness
  • Systematic bio-banking for future research activities

Infrastructure

Laboratory is equipped with high-ethroughput equipments to carry out biochemical analyses

Facilities Company
Electrochemiluminescence autoanalyzer (E411) Roche
HbA1c analyzer Bio-Rad
Electrolyte analyzer Caretium
ELISA reader Bio-Rad
Refrigerated centrifuge REMI
Table centrifuges, water baths, incubators, single channel and multi channel pipettes, magnetic stirrer, vortex machines, pH meter, weighing balance, water purification system etc.
Work is carried out by science graduates/ post graduates with training in Medical Laboratory Technology and Biotechnology with many years of experience.

Parameters

Clinical Chemistry

  • Glucose
  • Lipid Profile
  • Kidney function tests
  • Liver function tests
  • Urine Chemistry

Other markers of Cardio-metabolic disease

  • Insulin
  • HbA1c
  • Apo A1
  • Apo B

Specialized tests

  • Cytokines
  • Cotinine
  • Markers of Chronic Kidney Disease

Quality Assurance

Internal

  • Two levels of internal quality control samples run with every batch of samples
  • Westgard rules are followed for the rejection of runs

External

  • RIQAS program for Clinical Chemistry, Extended Lipids (ApoA1, Apo B) & HbA1c
  • UKNEQAS program for Insulin

lab-2Work from Jan 2011 till date …….

Studies Completed

  • Andhra Pradesh Parents & Children Study (APCAPS)
  • Cardio-metabolic Risk Reduction Surveillance Study (CARRS) – cohort 1 (Baseline, 2nd F-up), cohort-2
  • Dual Energy X-ray Absorptiometry (DEXA) study
  • ICMR Urban study
  • Solan Surveillance Study (SSS)
  • UDAY – Sonepat & Vizag
  • Salt study
  • Cytokine analysis

lab-3Bio-repository

  • Systematic storage of samples
  • Range of stored samples: Serum, Fluoride plasma, EDTA plasma, Whole blood, Buffy coat, RBCs, Spot Urine, Blood spots on filter paper
  • Biological samples from 14 different studies: IMS, DXA,CARRS, ICMR-Urban Study, UDAY, Solan Surveillance Study, Salt Study, etc.

Facilities

  • Ultra-low Deep freezers (-80° C): 28
  • Deep freezers (-20° C): 4
  • Refrigerators: 5

Maintenance

  • 24-hr power back-up
  • Temperature monitoring

Future Directions

  • Expansion of laboratory in terms of infrastructure and analysis carried out to better facilitate research at PHFI
  • Expand and better facilitate the bio-repository to meet the ever- increasing needs of ongoing and future studies

Our Practices

  • Standard Operating Procedures for  laboratory activities
  • Log of all activities maintained
  • Good Laboratory Practices followed
  • Electronic data transfer for majority of analysis
  • Periodic Evaluation and Re-training of lab staff
  • Regular calibration and maintenance of equipments